- NCBI Gene Id:
- Protein Name:
- C-C motif chemokine 5
- Alias Symbols:
- SISd, SCYA5, RANTES, TCP228, D17S136E, MGC17164, CCL5
- Replacement Item:
- This antibody may replace item sc-101501 from Santa Cruz Biotechnology.
- Description of Target:
- RABBIT ANTI HUMAN RANTES
- Protein Size (# AA):
- ELISA, WB
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express CCL5.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express CCL5.
- The immunogen for anti-CCL5 antibody: recombinant human RANTES
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG - lyophilised
- Reconstitution and Storage:
- Reconstitution: Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-CCL5 antibody - OASA08141
- Polyclonal IgG
- Application Info:
- This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Preservative Stabilisers: None present
Antiserum Preparation: Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
- Approx Protein Conc: IgG concentration 1.0 mg/ml after reconstitution
Buffer Solutions: Phosphate buffered saline pH7.4
- Target Reference:
- 1. Appay, V. et al. (2000) RANTES activates antigen-specific cytotoxic T lymphocytes in a mitogen-like manner through cell surface aggregation. Int. Immunol. 12: 1173-1182.
2. Dragic, T. et al. (1996) HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature. 381: 667-673.