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Catalog No: OKCD00144 (Formerly GWB-KBBOZ5)
Size:96WELLS
Price: $650.00
SKU
OKCD00144
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Datasheets/ManualsPrintable datasheet for CCL4 ELISA Kit (Rabbit) (OKCD00144)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)87-101%79-92%83-91%91-101%
EDTA plasma(n=5)86-94%88-96%82-96%94-105%
heparin plasma(n=5)88-98%78-99%79-105%92-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Beta (MIP1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Beta (MIP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Beta (MIP1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Macrophage Inflammatory Protein 1 Beta (MIP1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-9086
EDTA plasma(n=5)87-9491
heparin plasma(n=5)92-10497
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Beta (MIP1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Beta (MIP1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CCL4 Microplate96 Wells (12 x 8 Well Strips)
CCL4 Lyophilized Standard2 x 1 ng
100X Biotinylated CCL4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity< 5.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Beta (MIP1b). No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Beta (MIP1b) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCCL4
Gene Full Namechemokine (C-C motif) ligand 4
Alias SymbolsACT2;ACT-2;C-C motif chemokine 4;immune activation gene 2;immune activation protein 2;macrophage inflammatory protein 1-beta;MIP-1-beta;small-inducible cytokine A4.
NCBI Gene Id100008984
Protein NameC-C motif chemokine 4
Description of TargetMonokine with inflammatory and chemokinetic properties.
Uniprot IDP46632
Protein Accession #NP_001075665
Nucleotide Accession #NM_001082196.1
Protein Size (# AA)92
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