CCL3L1 Chemi-Luminescent ELISA Kit (Human) (OKCD03362)

Catalog No: OKCD03362

Size:96WELLS

Human CCL3L1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) level in the sample or standard.
• ELISA Kit Range: 6.86-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CCL3L1 Microplate: 96 Wells (12 x 8 Well strips)
  • CCL3L1 Lyophilized Standard: 2
  • 100X CCL3L1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL3L1|CCL3L3
• Gene Full Name: C-C motif chemokine ligand 3 like 1|C-C motif chemokine ligand 3 like 3
• Alias Symbols: 464.2;C-C motif chemokine 3-like 1;chemokine (C-C motif) ligand 3-like 1;chemokine (C-C motif) ligand 3-like 3;chemokine (C-C motif) ligand 3-like, centromeric;D17S1718;G0/G1 switch regulatory protein 19-2;G0S19-2;LD78;LD78BETA;LD78-beta(1-70);MIP1AP;PAT 464.2;SCYA3L;SCYA3L1;small inducible cytokine A3-like 1;Small-inducible cytokine A3-like 1;tonsillar lymphocyte LD78 beta protein.
• NCBI Gene Id: 414062|6349
• Protein Name: C-C motif chemokine 3-like 1
• Description of Target: Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1-infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5.
• Uniprot ID: P16619
• Protein Accession #: NP_001001437
• Nucleotide Accession #: NM_001001437