Ccl3 ELISA Kit (Rat) (OKBB01531)

Catalog No: OKBB01531

Size:96 wells

Ccl3 ELISA Kit (Rat) (OKBB01531) Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: The Aviva Quick Picokine™ Rat Ccl3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Rat Ccl3 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A24-A92. To measure Rat Ccl3, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The absorbance of the yellow product is linearly proportional to Rat Ccl3 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ccl3 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Aviva's ELISA Resource Center at https://www.Avivabio.com/elisa-technical-resource-center.
• ELISA Kit Range: 7.8 pg/ml - 500 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	18	54	267	18	56	248
  Standard deviation	1.08	3.61	15.75	1.11	4.48	14.88
  CV(%)	6	6.7	5.9	6.2	8	6

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Rat Ccl3 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Rat Ccl3 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Rat Ccl3 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	18	18	18	16	17	0.86	5%
  Sample 2	54	52	47	47	50	3.08	6.1%
  Sample 3	267	269	269	304	277	15.46	5.5%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
  O.D.	0.008	0.086	0.179	0.315	0.643	1.134	1.719	2.183

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <1 pg/ml
• Specificity: Natural and recombinant rat Ccl3
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A24-A92
• Description: The Quick Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat Ccl3 with <1 pg/ml sensitivity. Format: 96-well plate with removable strips.
  Compatible samples: cell culture supernatants, serum and plasma (heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.

Target Info

• Gene Symbol: CCL3
• Gene Full Name: C-C motif chemokine 3
• Alias Symbols: C-C motif chemokine 3;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;Small-inducible cytokine A3;Ccl3;Mip1a, Scya3;
• NCBI Gene Id: 25542
• Protein Name: C-C motif chemokine 3
• Description of Target: Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
• Uniprot ID: P50229
• Protein Accession #: NP_037157.2
• Nucleotide Accession #: NM_013025.2