CCL3 ELISA Kit (Dog) (OKCD02726)

Catalog No: OKCD02726

Size:96WELLS

Dog CCL3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Canis lupus|Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-105%	95-105%	79-91%	87-102%
  EDTA Plasma (n=5)	80-89%	78-102%	85-94%	81-101%
  Heparin Plasma (n=5)	78-101%	93-102%	78-101%	95-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Alpha (MIP1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Macrophage Inflammatory Protein 1 Alpha (MIP1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.81-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-90	84
  EDTA Plasma (n=5)	92-105	96
  Heparin Plasma(n=5)	90-97	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CCL3 Microplate: 96 Wells (12 x 8 Well strips)
  • CCL3 Lyophilized Standard: 2
  • 100X Biotinylated CCL3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 2.8 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Alpha (MIP1a). No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL3
• Alias Symbols: Small-inducible cytokine A3.
• Protein Name: C-C motif chemokine 3
• Description of Target: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5 (By similarity).
• Uniprot ID: Q68A92
• Protein Accession #: NP_001005251.2
• Nucleotide Accession #: NM_001005251.2
• Protein Size (# AA): 92