Catalog No: OKCD03751
Size:96WELLS
Price: $771.00
SKU
OKCD03751
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CCL3 Chemi-Luminescent ELISA Kit (Human) (OKCD03751)

Datasheets/ManualsPrintable datasheet for CCL3 Chemi-Luminescent ELISA Kit (Human) (OKCD03751)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)81-90%91-99%85-94%78-98%
EDTA Plasma (n=5)83-97%86-95%89-99%95-103%
Heparin Plasma (n=5)90-98%85-102%80-104%86-97%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Inflammatory Protein 1 Alpha (MIP1a) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-9794
EDTA Plasma (n=5)94-10198
Heparin Plasma (n=5)84-9990
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CCL3 Microplate96 Wells (12 x 8 Well strips)
CCL3 Lyophilized Standard2
100X CCL3 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity2.74 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCCL3
Gene Full NameC-C motif chemokine ligand 3
Alias SymbolsC-C motif chemokine 3;G0/G1 switch regulatory protein 19-1;G0S19-1;LD78ALPHA;macrophage inflammatory protein 1-alpha;MIP1A;MIP-1-alpha;PAT 464.1;SCYA3;SIS-beta;small inducible cytokine A3 (homologous to mouse Mip-1a);Small-inducible cytokine A3;tonsillar lymphocyte LD78 alpha protein.
NCBI Gene Id6348
Protein NameC-C motif chemokine 3
Description of TargetMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Uniprot IDP10147
Protein Accession #NP_002974
Nucleotide Accession #NM_002983
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