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Catalog No: OKCD03801
Size:96WELLS
Price: $800.00
SKU
OKCD03801
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CCL24 Chemi-Luminescent ELISA Kit (Human) (OKCD03801)

Datasheets/ManualsPrintable datasheet for CCL24 Chemi-Luminescent ELISA Kit (Human) (OKCD03801)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)94-103%82-91%94-102%90-104%
EDTA Plasma (n=5)88-102%83-98%97-105%84-99%
Heparin Plasma (n=5)82-92%79-89%80-101%78-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myeloid Progenitor Inhibitory Factor 2 (MPIF2) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)89-9892
EDTA Plasma (n=5)89-10394
Heparin Plasma (n=5)84-9689
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CCL24 Microplate96 Wells (12 x 8 Well strips)
CCL24 Lyophilized Standard2
100X CCL24 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity13.7 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCCL24
Gene Full NameC-C motif chemokine ligand 24
Alias SymbolsC-C motif chemokine 24;chemokine (C-C motif) ligand 24;Ckb-6;CK-beta-6;eosinophil chemotactic protein 2;eotaxin-2;MPIF2;MPIF-2;myeloid progenitor inhibitory factor 2;SCYA24;small inducible cytokine subfamily A (Cys-Cys), member 24;small-inducible cytokine A24.
NCBI Gene Id6369
Protein NameC-C motif chemokine 24
Description of TargetChemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
Uniprot IDO00175
Protein Accession #NP_002982
Nucleotide Accession #NM_002991
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