Catalog No: OKEH04246
Size:96T
Price: $525.00
SKU
OKEH04246
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CCL23 ELISA Kit (Human) (OKEH04246) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL23 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL23 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL23 captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.1% (n = 20)
Mean Inter-assay CV%: <= 7.4% (n = 20)
ELISA Kit Component
ComponentAmount
CCL23 Microplate96 Wells (12 x 8 Well Strips)
CCL23 Standard2 x 2 ng
Sample Diluent1 x 20 mL
CCL23 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human C-C motif chemokine 23
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCCL23
Gene Full NameC-C motif chemokine ligand 23
Alias SymbolsC6 beta-chemokine;C-C motif chemokine 23;chemokine (C-C motif) ligand 23;Ckb-8;CKb8;Ckb-8-1;CK-BETA-8;hmrp-2a;macrophage inflammatory protein 3;MIP3;MIP-3;MPIF-1;myeloid progenitor inhibitory factor 1;SCYA23;small inducible cytokine subfamily A (Cys-Cys), member 23;Small-inducible cytokine A23.
NCBI Gene Id6368
Protein NameP55773
Description of TargetC-C motif chemokine 23
Uniprot IDhttps://www.uniprot.org/uniprot/P55773
Protein Accession #NP_005055.3
Nucleotide Accession #NM_005064.5
Protein Size (# AA)120
Molecular Weight13 kDa
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