CCL23 Chemi-Luminescent ELISA Kit (Human) (OKCD03800)

Catalog No: OKCD03800

Size:96WELLS

Human CCL23 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	87-99%	78-99%	80-98%	92-99%
  EDTA Plasma (n=5)	97-105%	90-99%	91-98%	89-103%
  Heparin Plasma (n=5)	97-104%	97-104%	96-103%	84-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 1 (MPIF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 1 (MPIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myeloid Progenitor Inhibitory Factor 1 (MPIF1) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-91	86
  EDTA Plasma (n=5)	90-104	93
  Heparin Plasma (n=5)	82-98	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 1 (MPIF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 1 (MPIF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CCL23 Microplate: 96 Wells (12 x 8 Well strips)
  • CCL23 Lyophilized Standard: 2
  • 100X CCL23 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL23
• Gene Full Name: C-C motif chemokine ligand 23
• Alias Symbols: C6 beta-chemokine;C-C motif chemokine 23;chemokine (C-C motif) ligand 23;Ckb-8;CKb8;Ckb-8-1;CK-BETA-8;hmrp-2a;macrophage inflammatory protein 3;MIP3;MIP-3;MPIF-1;myeloid progenitor inhibitory factor 1;SCYA23;small inducible cytokine subfamily A (Cys-Cys), member 23;Small-inducible cytokine A23.
• NCBI Gene Id: 6368
• Protein Name: C-C motif chemokine 23
• Description of Target: Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23.
• Uniprot ID: P55773
• Protein Accession #: NP_005055
• Nucleotide Accession #: NM_005064