Catalog No: OKWB00363
Size:96 Wells
Price: $450.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-100%91-105%91-104%86-101%
EDTA Plasma (n=5)82-101%82-95%87-97%84-97%
Heparin Plasma (n=5)81-99%80-97%84-100%82-94%
ELISA Kit PrincipleAviva Systems Biology Ccl22 ELISA Kit (Rat) (OKWB00363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl22 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl22 captured in the well.
ELISA Kit Range15.625 - 1,000 pg/mL
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)91-10194
EDTA Plasma (n=5)88-10495
Heparin Plasma (n=5)88-10293
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
Ccl22 Microplate96 Wells (12 x 8 Well strips)
Ccl22 Lyophilized Standard2 x 1 ng
100X Ccl22 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity9.4 pg/mL

MiR-384 induces apoptosis and autophagy of non-small cell lung cancer cells through the negative regulation of Collagen a-1(X) chain gene. Biosci Rep. 39, (2019). 30442874

Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolCCL22
Alias SymbolsMdc, MGC108943, Scya22
Protein NameC-C motif chemokine
Uniprot IDQ9QZU1
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