CCL22 ELISA Kit (Rat) (OKWB00363)

Catalog No: OKWB00363

Size:96 Wells

Rat CCL22 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-100%	91-105%	91-104%	86-101%
  EDTA Plasma (n=5)	82-101%	82-95%	87-97%	84-97%
  Heparin Plasma (n=5)	81-99%	80-97%	84-100%	82-94%

• ELISA Kit Principle: Aviva Systems Biology Ccl22 ELISA Kit (Rat) (OKWB00363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl22 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl22 captured in the well.
• ELISA Kit Range: 15.625 - 1,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	91-101	94
  EDTA Plasma (n=5)	88-104	95
  Heparin Plasma (n=5)	88-102	93

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Ccl22 Microplate: 96 Wells (12 x 8 Well strips)
  • Ccl22 Lyophilized Standard: 2 x 1 ng
  • 100X Ccl22 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 9.4 pg/mL
• Publications:

  MiR-384 induces apoptosis and autophagy of non-small cell lung cancer cells through the negative regulation of Collagen a-1(X) chain gene. Biosci Rep. 39, (2019). 30442874

• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL22
• Alias Symbols: Mdc, MGC108943, Scya22
• Protein Name: C-C motif chemokine
• Uniprot ID: Q9QZU1