CCL21C Chemi-Luminescent ELISA Kit (Mouse) (OKCD05570)

Catalog No: OKCD05570

Size:96WELLS

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Secondary Lymphoid Tissue Chemokine (SLC) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CCL21C Microplate: 96 Wells (12 x 8 Well strips)
  • CCL21C Lyophilized Standard: 2
  • 100X CCL21C HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 1.31pg/mL

Target Info

• Gene Symbol: Ccl21b|Ccl21c|Ccl21d|Gm10591|Gm13304|LOC100041504|LOC100041593
• Gene Full Name: C-C motif chemokine 21c|chemokine (C-C motif) ligand 21B (leucine)|chemokine (C-C motif) ligand 21C (leucine)|chemokine (C-C motif) ligand 21D|predicted gene 10591|predicted gene 13304
• Alias Symbols: 6CKBAC;6CKBAC1;6Cki;6Ckine;AI159701;beta-chemokine exodus-2;C-C motif chemokine 21b;C-C motif chemokine 21c;c-C motif chemokine 21c-like;Ccl21b;Ccl21c;Ccl21-leu;chemokine (C-C motif) ligand 21C (leucine);CKb9;Exodus;exodus-2;Gm21541;S;Scya;Scya2;Scya21;Scya21a;Scya21b;Scya21c;secondary lymphoid organ chemokine;SLC;small inducible cytokine A21;small inducible cytokine A21c;small-inducible cytokine A21a;small-inducible cytokine A21b;Small-inducible cytokine A21c;TCA;TCA4;thymus-derived chemotactic agent 4.
• NCBI Gene Id: 100041504|100041593|100042493|100504239|100504346|100862177|65956
• Protein Name: C-C motif chemokine 21c
• Description of Target: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.
• Uniprot ID: P86793
• Protein Accession #: NP_001180595.1
• Nucleotide Accession #: NM_001193666.1