CCL21 Chemi-Luminescent ELISA Kit (Human) (OKCD03925)

Catalog No: OKCD03925

Size:96WELLS

Human CCL21 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-101%	86-94%	91-98%	91-99%
  EDTA Plasma (n=5)	79-95%	95-103%	78-99%	89-99%
  Heparin Plasma (n=5)	89-103%	89-96%	78-93%	92-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Secondary Lymphoid Tissue Chemokine (SLC) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-95	90
  EDTA Plasma (n=5)	92-101	95
  Heparin Plasma (n=5)	90-104	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CCL21 Microplate: 96 Wells (12 x 8 Well strips)
  • CCL21 Lyophilized Standard: 2
  • 100X CCL21 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL21
• Gene Full Name: C-C motif chemokine ligand 21
• Alias Symbols: 6Ckine;beta chemokine exodus-2;Beta-chemokine exodus-2;C-C motif chemokine 21;chemokine (C-C motif) ligand 21;CKb9;ECL;Efficient Chemoattractant for Lymphocytes;SCYA21;secondary lymphoid tissue chemokine;Secondary lymphoid-tissue chemokine;SLC;small inducible cytokine subfamily A (Cys-Cys), member 21;Small-inducible cytokine A21;TCA4.
• NCBI Gene Id: 6366
• Protein Name: C-C motif chemokine 21
• Description of Target: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
• Uniprot ID: Q6ICR7
• Protein Accession #: NP_002980
• Nucleotide Accession #: NM_002989