Catalog No: OKEH00168
Size:96T
Price: $501.00
SKU
OKEH00168
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CCL20 ELISA Kit (Human) : 96 Wells (OKEH00168)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CCL20 ELISA Kit (Human) (OKEH00168) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL20 captured in well.
ELISA Kit Range7.8-500pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 10.8% (n = 20)
ELISA Kit Component
ComponentAmount
CCL20 Microplate96 Wells (12 x 8 Well strips)
CCL20 Lyophilized Standard2
100X Biotinylated CCL20 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity3.5 pg/mL
SpecificityNatural and recombinant Human C-C motif chemokine 20
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCCL20
Gene Full NameC-C motif chemokine ligand 20
Alias Symbolsbeta chemokine exodus-1;Beta-chemokine exodus-1;CC chemokine LARC;C-C motif chemokine 20;chemokine (C-C motif) ligand 20;CKb4;Exodus;LARC;liver and activation-regulated chemokine;macrophage inflammatory protein 3 alpha;MIP-3a;MIP3A;MIP-3-alpha;SCYA20;small inducible cytokine subfamily A (Cys-Cys), member 20;small-inducible cytokine A20;ST38.
NCBI Gene Id6364
Protein NameP78556
Description of TargetC-C motif chemokine 20
Uniprot IDhttps://www.uniprot.org/uniprot/P78556
Protein Accession #NP_001123518
Nucleotide Accession #NM_001130046
Protein Size (# AA)96
Molecular Weight11 kDa
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