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Catalog No: OKCD05894
Size:96WELLS
Price: $850.00
SKU
OKCD05894
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Datasheets/ManualsPrintable datasheet for CCL2 ELISA Kit (Guinea Pig) (OKCD05894)
Product Info
Predicted Species ReactivityCavia porcellus|Guinea Pig
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte Chemotactic Protein 1 (MCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Monocyte Chemotactic Protein 1 (MCP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CCL2 Microplate96 Wells (12 x 8 Well strips)
CCL2 Lyophilized Standard2
100X CCL2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 6.8pg/mL
Gene SymbolCcl2
Gene Full Namechemokine (C-C motif) ligand 2
Alias SymbolsC-C motif chemokine 2;MCP1;MCP-1;Monocyte chemoattractant protein 1;monocyte chemoattractant protein-1;monocyte chemotactic protein 1;SCYA2;small-inducible cytokine A2.
NCBI Gene Id100135494
Protein NameC-C motif chemokine 2
Description of TargetChemotactic factor that attracts monocytes, but not neutrophils.
Uniprot IDQ08782
Protein Accession #NP_001166397.1
Nucleotide Accession #NM_001172926.1
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