CCL2 Chemi-Luminescent ELISA Kit (Rabbit) (OKCD03742)

Catalog No: OKCD03742

Size:96WELLS

Rabbit CCL2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-99%	89-98%	90-103%	82-101%
  EDTA Plasma (n=5)	79-94%	82-90%	80-88%	98-105%
  Heparin Plasma (n=5)	86-97%	94-101%	88-97%	80-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Monocyte Chemotactic Protein 1 (MCP1) level in the sample or standard.
• ELISA Kit Range: 54.9-40,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-101	94
  EDTA Plasma (n=5)	95-102	98
  Heparin Plasma (n=5)	93-105	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Ccl2 Microplate: 96 Wells (12 x 8 Well strips)
  • Ccl2 Lyophilized Standard: 2
  • 100X Ccl2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 54.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL2
• Gene Full Name: chemokine (C-C motif) ligand 2
• Alias Symbols: C-C motif chemokine 2;MCP-1;Monocyte chemoattractant protein 1;monocyte chemoattractant protein-1;monocyte chemotactic protein 1;small-inducible cytokine A2.
• NCBI Gene Id: 100009130
• Protein Name: C-C motif chemokine 2
• Description of Target: Chemotactic factor that attracts monocytes, but not neutrophils.
• Uniprot ID: P28292
• Protein Accession #: NP_001075763
• Nucleotide Accession #: NM_001082294