CCL2 Chemi-Luminescent ELISA Kit (Human) (OKCD03741)

Catalog No: OKCD03741

Size:96WELLS

Human CCL2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	98-105%	90-102%	82-102%
  EDTA Plasma (n=5)	79-102%	97-105%	80-101%	93-104%
  Heparin Plasma (n=5)	94-101%	79-95%	90-97%	83-94%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Monocyte Chemotactic Protein 1 (MCP1) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-88	81
  EDTA Plasma (n=5)	85-104	94
  Heparin Plasma (n=5)	83-94	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CCL2 Microplate: 96 Wells (12 x 8 Well strips)
  • CCL2 Lyophilized Standard: 2
  • 100X CCL2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 2.74 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL2
• Gene Full Name: C-C motif chemokine ligand 2
• Alias Symbols: C-C motif chemokine 2;chemokine (C-C motif) ligand 2;GDCF-2;HC11;HSMCR30;MCAF;MCP1;MCP-1;Monocyte chemoattractant protein 1;monocyte chemoattractant protein-1;monocyte chemotactic and activating factor;monocyte chemotactic protein 1;monocyte secretory protein JE;SCYA2;small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je);small inducible cytokine subfamily A (Cys-Cys), member 2;small-inducible cytokine A2;SMC-CF.
• NCBI Gene Id: 6347
• Protein Name: C-C motif chemokine 2
• Description of Target: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
• Uniprot ID: P13500
• Protein Accession #: NP_002973
• Nucleotide Accession #: NM_002982