Ccl2 Antibody (OASA08453)
|Predicted Species Reactivity||Mouse|
|Product Format||Purified IgG - lyophilised|
|Additional Information||Note: For long term storage, the addition of 0.09% sodium azide is recommended.|
N.B. For functional studies do not add azide.
|::||Preservative Stabilisers: None present|
Antiserum Preparation: Antisera to mouse MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
|::||Approx Protein Conc: IgG concentration 1.0 mg/ml|
Buffer Solutions: Phosphate buffered saline pH7.4
|Reconstitution and Storage||Reconstitution: Reconstitute with 0.1 ml distilled water|
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Replacement Item||This antibody may replace item sc-130328 from Santa Cruz Biotechnology.|
|Immunogen||The immunogen for anti-Ccl2 antibody: recombinant mouse MCP-1|
|Predicted Homology Based on Immunogen Sequence||Mouse|
|Datasheets/Manuals||Printable datasheet for anti-Ccl2 antibody - OASA08453|
|Application Info||ELISA: 0.5ug/ml|
Western Blotting: 0.1ug/ml - 0.2ug/ml
Functional Assays: 4.0ug/ml - 6.0ug/ml
|Target Reference||1. Tesch GH et. al. (1999) Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis. J Clin Invest. 103:73-80|
|Alias Symbols||JE, HC11, MCAF, MCP1, MCP-1, Scya2, Sigje, SMC-CF, AI323594, Ccl2|
|NCBI Gene Id||20296|
|Protein Name||C-C motif chemokine 2|
|Description of Target||RABBIT ANTI MOUSE MCP-1|
|Protein Accession #||NP_035463.1|
|Protein Size (# AA)||148|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express Ccl2.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express Ccl2.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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