CCL14/HCC-1 ELISA Kit (Human) (OKBB00501)

Catalog No: OKBB00501

Size:96 Wells

Human CCL14/HCC-1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human CCL14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL14 has been precoated onto 96-well plates. Standards(E.coli, T20-N93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCL14 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	34	163	521	36	182	534
  SD	1.33	7.66	33.87	1.84	12.56	38.45
  CV%	3.9	4.7	6.5	5.1	6.9	7.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human CCL14 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human CCL14 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human CCL14 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human CCL14
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: T20-N93
• Description: For quantitative detection of human CCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CCL14
• Gene Full Name: chemokine (C-C motif) ligand 14
• Alias Symbols: C-C motif chemokine 14, CC-1, CC-3, Chemokine (C-C motif) ligand 14, Chemokine CC-1/CC-3, Chemokine CC-3, CKB1, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, MCIF, NCC-2, NCC2, New CC chemokine 2, SCYA14, SCYL2, Small inducible cytokine subfamily A (Cys-Cys), member 14, Small-inducible cytokine A14, SY14
• NCBI Gene Id: 6358
• Protein Name: C-C motif chemokine 14
• Description of Target: Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
• Uniprot ID: Q16627
• Protein Accession #: NP_116738.1
• Nucleotide Accession #: NM_032962.4