CCL13/MCP4 ELISA Kit (Human) (OKBB00442)

Catalog No: OKBB00442

Size:96 Wells

Human CCL13/MCP4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human CCL13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL13 has been precoated onto 96-well plates. Standards(E.coli, Q24-T98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCL13 amount of sample captured in plate.
• ELISA Kit Range: 7.8 pg/ml - 500 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	50	191	325	54	223	356
  SD	2.15	8.98	20.15	3.13	14.05	27.77
  CV%	4.3	4.7	6.2	5.8	6.3	7.8

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human MCP4 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human MCP4 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human MCP4 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human CCL13
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: Q24-T98
• Description: For quantitative detection of human CCL13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CCL13
• Gene Full Name: chemokine (C-C motif) ligand 13
• Alias Symbols: C-C motif chemokine 13, CCL13, CCL13_HUMAN, Chemokine (C C motif) ligand 13, CK beta 10, CK-beta-10, CKb10, Macrophage/monocyte chemotactic protein 4, MCP-4, MGC17134, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC 1, NCC-1, NCC1, New CC chemokine 1, SCYA13, SCYL1, short chain, Small inducible cytokine A13, Small inducible cytokine A13 precursor, Small inducible cytokine subfamily A (Cys Cys) member 13, Small-inducible cytokine A13
• NCBI Gene Id: 6357
• Protein Name: C-C motif chemokine 13
• Description of Target: Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-α.
• Uniprot ID: Q99616
• Protein Accession #: NP_005399.1
• Nucleotide Accession #: NM_005408.2