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Catalog No: OKEH07614
Size:96T
Price: $725.00
SKU
OKEH07614
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CCKBR ELISA Kit (Human) (OKEH07614)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CCKBR ELISA Kit (Human) (OKEH07614) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCKBR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCKBR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCKBR captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=2.9% (n = 20)
Mean Inter-assay CV%: <=7.2% (n = 20)
ELISA Kit Component
ComponentAmount
CCKBR Microplate96 Wells (12 x 8 Well strips)
CCKBR Lyophilized Standard2
100X Biotinylated CCKBR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
::Subcellular Location: Cell membrane Multi-pass membrane protein
::Unigene: Hs.203
SMR: P32239
String: 9606.ENSP00000335544
::KEGG: hsa:887
MIM: 118445
Pfam: PF00001
Reconstitution and StorageStore as indicated in product manual.
Sensitivity39.13pg/mL
SpecificityNatural and recombinant Human Gastrin/cholecystokinin type B receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCCKBR
Gene Full Namecholecystokinin B receptor
Alias SymbolsCCK2 receptor;CCK2R;CCK-B;CCK-B receptor;cholecystokinin-2 receptor;GASR;gastrin receptor;gastrin/cholecystokinin type B receptor.
NCBI Gene Id887
Protein NameP32239
Description of TargetGastrin/cholecystokinin type B receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P32239
Protein Accession #NP_001304958.1
Nucleotide Accession #NM_001318029.1
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