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CCKAR ELISA Kit (Rat) (OKDD00625)

96 Wells
$580.00
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Gene Symbol:
CCKAR
Official Gene Full Name:
cholecystokinin A receptor
NCBI Gene Id:
24889
Alias Symbols:
CCK1-R, CCK-AR, CCK-A receptor, Cholecystokinin-1 receptor, Cholecystokinin receptor type A
Description of Target:
G-protein coupled receptor activated by cholecystokinin; may be involved in food intake and insulin regulation [rgd, feb 2006]
Protein Name:
cholecystokinin receptor type A
Swissprot Id:
P30551
Protein Accession #:
NP_036820.1
Nucleotide Accession #:
NM_012688.3
Predicted Species Reactivity:
Rat
Sample Type:
Serum, plasma, tissue homogenates
Sensitivity:
<0.079 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

Kit Duration:
~4 Hours
Kit Principle:
Aviva Systems Biology CCKAR ELISA Kit (Rat) (OKDD00625) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for CCKAR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCKAR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CCKAR captured in well.
Kit Component:
ComponentAmount
Anti-CCKAR Microplate96 Wells (12 x 8 Well Strips)
CCKAR Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CCKAR Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CCKAR.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CCKAR.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of CCKAR. No significant cross-reactivity or interference between CCKAR and analogues was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
50 kDa
Protein Size (# AA):
444

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