Size:96 Wells
$689.00
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SKU
OKDD00625
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CCKAR ELISA Kit (Rat) (OKDD00625)

Catalog#: OKDD00625
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
This product is Genway GWB-KBBNT2
More Information
Predicted Species ReactivityRat
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~4 Hours
Kit PrincipleAviva Systems Biology CCKAR ELISA Kit (Rat) (OKDD00625) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for CCKAR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCKAR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CCKAR captured in well.
Kit Range0.156-10 ng/mL
Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

Kit Component
ComponentAmount
Anti-CCKAR Microplate96 Wells (12 x 8 Well Strips)
CCKAR Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CCKAR Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates
Sensitivity<0.079 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityThis assay has high sensitivity and excellent specificity for detection of CCKAR. No significant cross-reactivity or interference between CCKAR and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolCCKAR
Official Gene Full Namecholecystokinin A receptor
Alias SymbolsCCK1-R, CCK-AR, CCK-A receptor, Cholecystokinin-1 receptor, Cholecystokinin receptor type A
NCBI Gene Id24889
Protein Namecholecystokinin receptor type A
Description of TargetG-protein coupled receptor activated by cholecystokinin; may be involved in food intake and insulin regulation [rgd, feb 2006]
Swissprot IdP30551
Protein Accession #NP_036820.1
Nucleotide Accession #NM_012688.3
Protein Size (# AA)444
Molecular Weight50 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CCKAR.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CCKAR.
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