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Cckar Antibody (OAEB02153)

100 ug
$349.00
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Gene Symbol:
Cckar
NCBI Gene Id:
12425
Protein Name:
Cholecystokinin receptor type A
Swissprot Id:
O08786
Protein Accession #:
NP_033957.1
Alias Symbols:
Cckar, cholecystokinin A receptor, AW106902
Replacement Item:
This antibody may replace item sc-16172 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
ELISA, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Cckar.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Cckar.
Predicted Species Reactivity:
Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 86%; Guinea Pig: 91%; Horse: 90%; Human: 91%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Complete computational species homology data:
Cckar Antibody (OAEB02153)
Peptide Sequence:
C-KFDASQKKSAKEKR
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-Cckar antibody - OAEB02153
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J. Int J Endocrinol. 2009;2009:875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3ug/ml.
Target Reference:
* Bi S, Scott KA, Kopin AS, Moran TH.; Differential roles for cholecystokinin a receptors in energy balance in rats and mice.; Endocrinology. 2004 Aug;145(8):3873-80. Epub 2004 May 3.; Pubmed 15123537

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