CCK4 Antibody - N-terminal region (OAAB17250)

Catalog No: OAAB17250

Size:400ul

Western blot analysis of CCK4 (arrow) using rabbit polyclonal CCK4 Antibody (N-term) (Cat.# OAAB17250). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).

PTK7 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Flow cytometric analysis of NCI-H460 cells using CCK4 (PTK7) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Precautions: CCK4 (PTK7) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis
  Cellular Location: Membrane; Single-pass type I membrane protein. Cell junction. Note=Co-localizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells
  Tissue Location: Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CCK4 (PTK7) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 amino acids from the N-terminal region of human CCK4 (PTK7).
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  PTK7 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: FC: 1:10~50
  IHC-P: 1:50~100
  WB: 1:1000

Target Info

• Gene Symbol: PTK7
• Alias Symbols: CCK4, CCK-4
• NCBI Gene Id: 5754
• Protein Name: Inactive tyrosine-protein kinase 7
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q13308
• Protein Accession #: NP_001257327.1
• Molecular Weight: 118392 Da