CCK ELISA Kit (Pig | Swine) (OKCA02198)

Catalog No: OKCA02198

Size:96 Wells

Pig CCK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Pig | Swine
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	97	93-100
  Serum (n=4)	1:2	104	101-107
  Serum (n=4)	1:4	91	85-97
  Serum (n=4)	1:8	90	87-93

• ELISA Kit Principle: Aviva Systems Biology CCK ELISA Kit (Pig | Swine) (OKCA02198) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCK has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CCK captured in well.
• ELISA Kit Range: 1.56 pg/mL-100 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	85	81-89
  EDTA plasma (n=4)	92	87-98

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-CCK Microplate: 96 Wells (12 x 8 Well strips)
  • CCK Lyophilized Standard: 2 x 100 pg
  • 100X Biotinylated CCK Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.39 pg/mL.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCK
• NCBI Gene Id: 397468
• Protein Name: Cholecystokinin
• Description of Target: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
• Uniprot ID: P01356
• Protein Accession #: NP_999402
• Nucleotide Accession #: NM_214237