CCK ELISA Kit (Pig) (OKEH07480)

Catalog No: OKEH07480

Size:96T

Pig CCK ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	105-115% (n = 20)	84-94% (n = 20)	106-116% (n = 20)	93-105% (n = 20)
  EDTA plasma(n=5)	102-113% (n = 20)	97-109% (n = 20)	112-121% (n = 20)	86-96% (n = 20)
  heparin plasma(n=5)	103-113% (n = 20)	97-107% (n = 20)	93-105% (n = 20)	92-105% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology CCK ELISA Kit (OKEH07480) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-CCK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CCK and incubated. The CCK found in the sample or standards competes with the biotinylated CCK for limited binding sites on the immobilized anti-CCK antibody. Excess unbound biotinylated CCK and sample or standard CCK is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CCK captured in the well and inversely proportional to the amount of CCK which was contained in the sample or standard.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.8% (n = 20)
  Mean Inter-assay CV%: <=11.5% (n = 20)
• ELISA Kit Component:
  • Anti-CCK Microplate: 96 Wells (12 x 8 Well strips)
  • CCK Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X CCK-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
  Subcellular Location: Secreted
  Unigene: Ssc.717
  String: 9823.ENSSSCP00000012023
  KEGG: ssc:397468
  Pfam: PF00918
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 7.9pg/mL
• Specificity: Natural and recombinant Pig Cholecystokinin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: CCK
• Gene Full Name: cholecystokinin
• Alias Symbols: cholecystokinin.
• NCBI Gene Id: 397468
• Protein Name: P01356
• Description of Target: Cholecystokinin
• Uniprot ID: https://www.uniprot.org/uniprot/P01356
• Protein Accession #: NP_999402.1
• Nucleotide Accession #: NM_214237.2