Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH00596
Size:96T
Price: $650.00
SKU
OKEH00596
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

CCK ELISA Kit (Human) : 96 Wells (OKEH00596)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH00596
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CCK ELISA Kit (Human) (OKEH00596) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CCK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CCK and incubated. The CCK found in the sample or standards competes with the biotinylated CCK for limited binding sites on the immobilized anti-human CCK antibody. Excess unbound biotinylated CCK and sample or standard CCK is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CCK captured in the well and inversely proportional to the amount of CCK which was contained in the sample or standard.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CCK Microplate96 Wells (12 x 8 Well strips)
CCK Lyophilized Standard2
Sample Diluent1 x 20 mL
100X CCK-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Cholecystokinin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCCK
Gene Full Namecholecystokinin
Alias Symbolscholecystokinin;cholecystokinin triacontatriapeptide;prepro-cholecystokinin.
NCBI Gene Id885
Protein NameP06307
Description of TargetCholecystokinin
Uniprot IDhttps://www.uniprot.org/uniprot/P06307
Protein Accession #NP_000720.1
Nucleotide Accession #NM_000729.5
Protein Size (# AA)115
Molecular Weight12 kDa
Write Your Own Review
You're reviewing:CCK ELISA Kit (Human) : 96 Wells (OKEH00596)
Your Rating
We found other products you might like!