CCDC80 ELISA Kit (Human) (OKDD00171)

Catalog No: OKDD00171 (Formerly GWB-KBBCW5)

Size:96 Wells

Human CCDC80 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology CCDC80 ELISA Kit (Human) (OKDD00171) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for CCDC80 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCDC80 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CCDC80 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CCDC80 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CCDC80 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-CCDC80 Microplate: 96 Wells (12 x 8 Well Strips)
  • CCDC80 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X CCDC80 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.053 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of CCDC80. No significant cross-reactivity or interference between CCDC80 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCDC80
• Gene Full Name: coiled-coil domain containing 80
• Alias Symbols: CL2;coiled-coil domain-containing protein 80;down-regulated by oncogenes protein 1;DRO1;LINC01279;long intergenic non-protein coding RNA 1279;nuclear envelope protein okuribin;okuribin;SSG1;steroid sensitive gene 1;TCONS_00006930;up-regulated in BRS-3 deficient mouse homolog;URB.
• NCBI Gene Id: 151887
• Protein Name: Coiled-coil domain-containing protein 80
• Description of Target: Promotes cell adhesion and matrix assembly.
• Uniprot ID: Q76M96
• Protein Accession #: NP_955805.1
• Nucleotide Accession #: NM_199511.2
• Protein Size (# AA): 950
• Molecular Weight: 108 kDa