Catalog No: OKCD00587 (Formerly GWB-KBBLZ5)
Size:96WELLS
Price: $675.00
SKU
OKCD00587
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Datasheets/ManualsPrintable datasheet for CCDC80 ELISA Kit (Human) (OKCD00587)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-101%93-103%78-93%78-93%
EDTA Plasma (n=5)93-105%92-101%98-105%97-104%
Heparin Plasma (n=5)94-103%82-94%99-105%78-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coiled Coil Domain Containing Protein 80 (CCDC80). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coiled Coil Domain Containing Protein 80 (CCDC80). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coiled Coil Domain Containing Protein 80 (CCDC80), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coiled Coil Domain Containing Protein 80 (CCDC80) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)79-9692
EDTA plasma(n=5)82-10495
heparin plasma(n=5)96-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coiled Coil Domain Containing Protein 80 (CCDC80) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coiled Coil Domain Containing Protein 80 (CCDC80) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CCDC80 Microplate96 Wells (12 x 8 Well Strips)
CCDC80 Lyophilized Standard2 x 10 ng
100X Biotinylated CCDC80 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Coiled Coil Domain Containing Protein 80 (CCDC80). No significant cross-reactivity or interference between Coiled Coil Domain Containing Protein 80 (CCDC80) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCCDC80
Gene Full Namecoiled-coil domain containing 80
Alias SymbolsCL2;coiled-coil domain-containing protein 80;down-regulated by oncogenes protein 1;DRO1;LINC01279;long intergenic non-protein coding RNA 1279;nuclear envelope protein okuribin;okuribin;SSG1;steroid sensitive gene 1;TCONS_00006930;up-regulated in BRS-3 deficient mouse homolog;URB.
NCBI Gene Id151887
Protein NameCoiled-coil domain-containing protein 80
Description of TargetPromotes cell adhesion and matrix assembly.
Uniprot IDQ76M96
Protein Accession #NP_955805
Nucleotide Accession #NM_199511.2
Protein Size (# AA)950
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