Catalog No: OCOA05690
Size:20UG
Price: $179.00
SKU
OCOA05690
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CCDC112 Protein Lysate (OCOA05690)

Datasheets/ManualsPrintable datasheet for CCDC112 Protein Lysate (OCOA05690)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCCDC112 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCCDC112
Gene Full Namecoiled-coil domain containing 112
Alias Symbolscoiled-coil domain-containing protein 112;MBC1;mutated in bladder cancer 1;mutated in bladder cancer protein 1.
NCBI Gene Id153733
Protein NameNM_152549, NP_689762, Q8NEF3
Description of TargetCoiled-coil domain-containing protein 112, coiled-coil domain-containing protein 112 isoform 2 [Homo sapiens], Homo sapiens coiled-coil domain containing 112 (CCDC112), transcript variant 2, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q8NEF3
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_689762
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_152549
Molecular Weight53.5 kDa
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