CCBL1 ELISA Kit (Rat) (OKEH04184)

Catalog No: OKEH04184

Size:96T

Rat CCBL1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ccbl1 ELISA Kit (Rat) (OKEH04184) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccbl1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccbl1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccbl1 captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Component:
  • Ccbl1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ccbl1 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Ccbl1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Rat Kynurenine--oxoglutarate transaminase 1, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Kyat1
• Gene Full Name: kynurenine aminotransferase 1
• Alias Symbols: Ccbl1;cysteine conjugate-beta lyase 1;cysteine conjugate-beta lyase, cytoplasmic;cysteine-S-conjugate beta-lyase;glutamine transaminase K;glutamine--phenylpyruvate transaminase;Gtk;Kat1;KatI;Kynurenine aminotransferase 1;kynurenine aminotransferase I;kynurenine aminotransferase/glutamine transaminase K;kynurenine--oxoglutarate transaminase 1;kynurenine--oxoglutarate transaminase 1, mitochondrial;kynurenine--oxoglutarate transaminase I.
• NCBI Gene Id: 311844
• Protein Name: Q08415
• Description of Target: Kynurenine--oxoglutarate transaminase 1, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/Q08415
• Protein Accession #: NP_001013182.3
• Nucleotide Accession #: NM_001013164.3
• Protein Size (# AA): 457
• Molecular Weight: 52 kDa