Catalog No: OKCD08552
Size:96WELLS
Price: $800.00
SKU
OKCD08552
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for CCBL1 ELISA Kit (Human) (OKCD08552) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 78.12-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Conjugate Beta Lyase, Cytoplasmic (CCbL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 27pg/mL |
Gene Symbol | KYAT1 |
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Gene Full Name | kynurenine aminotransferase 1 |
Alias Symbols | beta-lysase, kidney;CCBL1;cysteine conjugate beta lyase 1;cysteine conjugate-beta lyase, cytoplasmic;cysteine conjugate-beta lyase; cytoplasmic (glutamine transaminase K, kyneurenine aminotransferase);cysteine-S-conjugate beta-lyase;glutamine transaminase K;glutamine-phenylpyruvate aminotransferase;glutamine--phenylpyruvate transaminase;GTK;KAT1;KATI;kyneurenine aminotransferase;Kynurenine aminotransferase 1;kynurenine aminotransferase I;kynurenine--oxoglutarate transaminase 1;kynurenine--oxoglutarate transaminase I. |
NCBI Gene Id | 883 |
Protein Name | Kynurenine--oxoglutarate transaminase 1 |
Description of Target | Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Metabolizes the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites. Catalyzes the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond. |
Uniprot ID | Q16773 |
Protein Accession # | NP_001116143.1 |
Nucleotide Accession # | NM_001122671.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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