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Catalog No: OPCA04068
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OPCA04068
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CBS Recombinant Protein (Human) (OPCA04068)

Datasheets/ManualsPrintable datasheet for OPCA04068
Product Info
Predicted Species ReactivityHuman
Product FormatLiquid or Lyophilized powder
Additional InformationSpecies Specificity Detail: Homo sapiens (Human)
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
FormulationTris-base, 50% glycerol
PurityGreater than 90% as determined by SDS-PAGE.
Protein SequenceMPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWIRPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIEPTSGNTGIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKKPWWWHLR
Storage BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
SourceYeast
Protein Range1-413 aa
TagN-terminal 6xHis-tagged
ReferenceHuman cystathionine beta-synthase cDNA sequence, alternative splicing and expression in cultured cells.Kraus J.P., Le K., Swaroop M., Ohura T., Tahara T., Rosenberg L.E., Roper M.D., Kozich V.Hum. Mol. Genet. 2:1633-1638(1993)
Gene SymbolCBS
Gene Full Namecystathionine-beta-synthase
Alias SymbolsBeta-thionase, Serine sulfhydrase
NCBI Gene Id875
Protein NameCystathionine beta-synthase
Description of TargetHydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine.
Uniprot IDP35520
Protein Accession #NP_000062
Nucleotide Accession #NM_000071
Protein Size (# AA)1-413 aa
Molecular Weight47.4 kDa
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