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Catalog No: OKCD02428
Size:96 Wells
Price: $700.00
SKU
OKCD02428
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration3 Hours
ELISA Kit PrincipleAviva Systems Biology Cbs ELISA Kit (Mouse) (OKCD02428) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cbs has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Cbs is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Cbs captured in well.
ELISA Kit Range0.156-10 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CBS was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CBS was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
ELISA Kit Component
ComponentAmount
Anti-CBS Microplate96 Wells (12 x 8 Well strips)
CBS Lyophilized Standard2
100X Biotinylated CBS Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cystathionine Beta Synthase (CbS). No significant cross-reactivity or interference between Cystathionine Beta Synthase (CbS) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCBS
Gene Full Namecystathionine beta-synthase
Alias SymbolsHI, HIP4, AI047524, AI303044
NCBI Gene Id12411
Protein NameCystathionine beta-synthase
Description of TargetHydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
Uniprot IDQ91WT9
Protein Accession #NP_001258282.1
Nucleotide Accession #NM_001271353.1
Protein Size (# AA)561
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