CBS Antibody - N-terminal region : HRP (ARP45746_T100-HRP)

Catalog No: ARP45746_T100-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: IHC, WB
• Additional Information: IHC Information: Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CBS
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 86%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: RCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNE
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CBS (ARP45746_T100-HRP) antibody is Catalog # AAP45746 (Previous Catalog # AAPP26703)
• Reference: Urreizti,R., (2006) J. Hum. Genet. 51 (4), 305-313
• Publications:

  Fernandes, V. S. et al. Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck. J. Urol. 189, 1567-73 (2013). IHC, Bovine, Human, Dog, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish, Yeast 23063804

  Markand, S. et al. Cystathionine beta synthase expression in mouse retina. Curr. Eye Res. 38, 597-604 (2013). IHC, Bovine, Human, Dog, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish, Yeast 23470016

  Dahlhoff, C. et al. Hepatic methionine homeostasis is conserved in C57BL/6N mice on high-fat diet despite major changes in hepatic one-carbon metabolism. PLoS One 8, e57387 (2013). WB, Bovine, Human, Dog, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish, Yeast 23472083

  Geillinger, K. E. et al. Hepatic metabolite profiles in mice with a suboptimal selenium status. J. Nutr. Biochem. 25, 914-22 (2014). WB, Bovine, Human, Dog, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish, Yeast 24917045

  Yamane, S., Kanno, T., Nakamura, H., Fujino, H. & Murayama, T. Hydrogen sulfide-mediated regulation of contractility in the mouse ileum with electrical stimulation: Roles of l-cysteine, cystathionine β-synthase, and K(+) channels. Eur. J. Pharmacol. 740, 112-20 (2014). WB, Bovine, Human, Dog, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish, Yeast 25008073

  Dahlhoff, C. et al. Methyl-donor supplementation in obese mice prevents the progression of NAFLD, activates AMPK and decreases acyl-carnitine levels. Mol. Metab. 3, 565-80 (2014). WB, Bovine, Human, Dog, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish, Yeast 25061561

  Porteus, C. S. et al. The role of hydrogen sulphide in the control of breathing in hypoxic zebrafish (Danio rerio). J. Physiol. 592, 3075-88 (2014). IHC, Bovine, Human, Dog, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish, Yeast 24756639

Target Info

• Gene Symbol: CBS
• Gene Full Name: Cystathionine-beta-synthase
• Alias Symbols: CBSL, HIP4
• NCBI Gene Id: 875
• Protein Name: Cystathionine beta-synthase
• Description of Target: CBS is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.The protein encoded by this gene is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.
• Uniprot ID: P35520
• Protein Accession #: NP_000062
• Nucleotide Accession #: NM_000071
• Protein Size (# AA): 551
• Molecular Weight: 60kDa
• Protein Interactions: UBASH3A; UBE2I; PSMA1; PRKAG1; PIN1; EHHADH; CBS; SUMO1; UBC; NEDD8; PIAS3; RANBP9; PIAS1; CBX4; SRP14; RAD23B; PKM; NUCB1; IREB2; HSF1; LRSAM1; C14orf142; UBA5; IPO4; GOPC; PARP6; NPLOC4; DERA; ERO1L; DBNL; GPN1; PDLIM5; PLIN3; UBA2; GPSM1; COL4A3BP; UGP