Catalog No: OCOA08153
Size:20UG
Price: $275.00
SKU
OCOA08153
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CBLL1 Protein Lysate (OCOA08153)

Datasheets/ManualsPrintable datasheet for CBLL1 Protein Lysate (OCOA08153)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCBLL1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCBLL1
Gene Full NameCbl proto-oncogene like 1
Alias SymbolsCas-Br-M (murine) ecotropic retroviral transforming sequence-like 1;casitas B-lineage lymphoma-transforming sequence-like protein 1;Cbl proto-oncogene like 1, E3 ubiquitin protein ligase;Cbl proto-oncogene, E3 ubiquitin protein ligase-like 1;c-Cbl-like protein 1;E3 ubiquitin-protein ligase Hakai;E-cadherin binding protein E7;HAKAI;RING finger protein 188;RING-type E3 ubiquitin transferase Hakai;RNF188.
NCBI Gene Id79872
Protein NameNM_024814, NP_079090, Q75N03
Description of TargetE3 ubiquitin-protein ligase Hakai, E3 ubiquitin-protein ligase Hakai isoform 1 [Homo sapiens], Homo sapiens Cbl proto-oncogene like 1 (CBLL1), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q75N03
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_079090
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_024814
Molecular Weight54.3 kDa
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