CaV3.1 Antibody (OASE00253)

Catalog No: OASE00253

Size:100ug

Western Blot analysis of Rat brain membrane lysate showing detection of mGluR5 Glutamate Receptor protein using Mouse Anti-mGluR5 Glutamate Receptor Monoclonal Antibody, Clone S75-33. Primary Antibody: Mouse Anti-mGluR5 Glutamate Receptor Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-33. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) mGluR1/5 Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S178A-9
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF
• Additional Information: Background Info: Detects ~<200kDa. Does not cross-react with Cav3.2
  Scientific Background: Calcium channel CaV3.1 (a1G) is a low-voltage-activated T-type calcium channel. Such T-type channels are expressed throughout the body. In the heart, they may be involved in pacemaker current. In neurons, these channels may play a secondary pacemaker role (3). With the ubiquitous expression, it is not surprising that alterations in channel function have been implicated in disease. Drugs that act to block T-type calcium channels are used as anti-hypertensives, antiepileptic's, and blocking of T-type calcium channels may be involved in the action of some anesthetics and antipsychotics as well (3). Much remains to be determined about the precise cellular localization, in vivo physiological roles, roles in disease states and possible routes to modulate their structure/function to ameliorate effects of disease.
  Certificate of Analysis: 1ug/mL was sufficient for detection of Cav3.1 in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~<200kDa. Does not cross-react with Cav3.2.
• Dilution: WB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA.
  2. www.iochannels.org
  3. Perez-Reyes E. (2003). Physiol. Rev., 83: 117-161.
  
• Description: Calcium channel CaV3.1 (a1G) is a low-voltage-activated T-type calcium channel. Such T-type channels are expressed throughout the body. In the heart, they may be involved in pacemaker current. In neurons, these channels may play a secondary pacemaker role (1). With the ubiquitous expression, it is not surprising that alterations in channel function have been implicated in disease. Drugs that act to block T-type calcium channels are used as anti-hypertensives, antiepileptic's, and blocking of T-type calcium channels may be involved in the action of some anesthetics and antipsychotics as well (1). Much remains to be determined about the precise cellular localization, in vivo physiological roles, roles in disease states and possible routes to modulate their structure/function to ameliorate effects of disease.

Target Info

• Gene Symbol: Cav3.1
• Gene Full Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
• Alias Symbols: CACNA1G, CaV T1, Cav3 1, cav3 1c, KIAA1123, MGC117234, NBR13, voltage gated calcium channel cav3.1, calcium channel voltage dependent alpha 1G subunit, calcium channel voltage dependent T type alpha 1G subunit, calcium channel voltage dependent T type alpha1G subunit, voltage dependent calcium channel alpha 1G subunit isoform 11, voltage dependent T type calcium channel subunit alpha 1G, Voltage gated calcium channel subunit alpha Cav3 1
• NCBI Gene Id: 12291
• Uniprot ID: Q9WUT2
• Protein Accession #: NP_001106284.1