CaV1.3 Antibody : HRP (OASE00151-HRP)

Catalog No: OASE00151-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: S48A-9
• Isotype: IgG2a Kappa
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IP, ICC, IF
• Additional Information: Background Info: Detects ~250kDa. No cross-reactivity against Cav1.2
  Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
  There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
  Specifically, CaV1.3, also known as the calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), is a human gene. CaV1.3 subunits are primarily expressed in neurons and neuroendocine cells. Some studies suggest however that CaV1.3 is also found in the atria, and may figure prominently in atrial arrhythmias (3). CaV1.3 also carries the primary sensory receptors of the mammalian cochlea, and are also expressed in the electromotile outer hair cells (4).
  
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Cav1.3 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 859-875 of rat Cav1.3, accession number P27732
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA.
  2. www.iochannels.org
  3. Zhang Z., et al. (2005) Circulation 112: 1936-1944.
  4. Johnson S.L. and Marcotti W. (2008) The Journal of
  Physiology. 586: 1029-1042.

Target Info

• Gene Symbol: Cacna1d
• Gene Full Name: calcium channel, voltage-dependent, L type, alpha 1D subunit
• NCBI Gene Id: 29716
• Uniprot ID: P27732
• Protein Accession #: NP_058994.1