Catalog No: OKEH03907
Size:96T
Price: $625.00
SKU
OKEH03907
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CAST ELISA Kit (Pig) (OKEH03907) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAST has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAST is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAST captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
CAST Microplate96 Wells (12 x 8 Well strips)
CAST Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CAST Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity28 pg/mL
SpecificityNatural and recombinant Pig Calpastatin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCAST
Gene Full Namecalpastatin
Alias SymbolsCalpain inhibitor.
NCBI Gene Id397135
Protein NameP12675
Description of TargetCalpastatin
Uniprot IDhttps://www.uniprot.org/uniprot/P12675
Protein Accession #NP_999232.1
Nucleotide Accession #NM_214067.1
Protein Size (# AA)713
Molecular Weight713 kDa
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