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Catalog No: OKCD02423
Size:96WELLS
Price: $700.00
SKU
OKCD02423
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Datasheets/ManualsPrintable datasheet for CAST ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpastatin (CAST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpastatin (CAST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpastatin (CAST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calpastatin (CAST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpastatin (CAST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpastatin (CAST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CAST Microplate96 Wells (12 x 8 Well strips)
CAST Lyophilized Standard2
100X Biotinylated CAST Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calpastatin (CAST). No significant cross-reactivity or interference between Calpastatin (CAST) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCAST
Gene Full Namecalpastatin
Alias SymbolsCalpain inhibitor, Calpastatin
NCBI Gene Id12380
Protein NameCalpastatin
Description of TargetSpecific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Uniprot IDP51125
Protein Accession #NP_001288082.1
Nucleotide Accession #NM_001301153.1
Protein Size (# AA)788
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