CASR Antibody (OAGA01562)

Catalog No: OAGA01562

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
OAGA01562 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using Calcium Sensing Receptor (OAGA01562) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calcium Sensing Receptor (OAGA01562) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calcium Sensing Receptor. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: CASR
• Gene Full Name: calcium-sensing receptor
• Alias Symbols: CAR, FHH, FIH, HHC, EIG8, HHC1, NSHPT, PCAR1, hCasR, GPRC2A, HYPOC1
• NCBI Gene Id: 846
• Description of Target: The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.
• Uniprot ID: P41180