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Datasheets/Manuals | Printable datasheet for Caspase-8 (P18) Polyclonal Antibody (OABB00267) |
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Tested Species Reactivity | Human, Rat |
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Predicted Species Reactivity | Human|Mouse|Rat |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Caspase 8 is a caspase protein. It most likely acts upon caspase 3. This gene encodes a member of the cysteine-aspartic acid protease(caspase) family. The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1beta converting enzyme(ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex(DISC). This gene contains at least 11 exons spanning approximately 30kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8. Caspase-8 deficiency in humans is compatible with normal development and shows that caspase-8 has a postnatal role in immune activation of naive lymphocytes. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse |
Reference | 1. Grenet, J.; Teitz, T.; Wei, T.; Valentine, V.; Kidd, V. J. : Structure and chromosome localization of the human CASP8 gene. Gene 226: 225-232, 1999. 2. Chun, H. J.; Zheng, L.; Ahmad, M.; Wang, J.; Speirs, C. K.; Siegel, R. M.; Dale, J. K.; Puck, J.; Davis, J.; Hall, C. G.; Skoda-Smith, S.; Atkinson, T. P.; Straus, S. E.; Lenardo, M. J. : Pleiotropic defects in lymphocyte activation caused by caspase-8 mutations lead to human immunodeficiency. Nature 419: 395-399, 2002. |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Caspase-8(CASP8) detection. Tested with WB in Human;Mouse;Rat. |
Gene Symbol | CASP8 |
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Gene Full Name | caspase 8 |
Alias Symbols | ALPS2B;apoptotic cysteine protease;apoptotic protease Mch-5;CAP4;Casp-8;caspase 8, apoptosis-related cysteine peptidase;caspase 8, apoptosis-related cysteine protease;caspase-8;FADD-homologous ICE/CED-3-like protease;FADD-like ICE;FLICE;ICE-like apoptotic protease 5;MACH;MACH-alpha-1/2/3 protein;MACH-beta-1/2/3/4 protein;MCH5;MORT1-associated ced-3 homolog. |
NCBI Gene Id | 841 |
Protein Name | Caspase-8 |
Description of Target | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. |
Uniprot ID | Q14790 |
Molecular Weight | 55391 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Caspase-8 (P18) Polyclonal Antibody (OABB00267)"?
The tested species reactivity for this item is "Human, Rat". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Caspase-8 (P18) Polyclonal Antibody (OABB00267)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "Caspase-8 (P18) Polyclonal Antibody (OABB00267)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Caspase-8 (P18) Polyclonal Antibody (OABB00267)"?
This target may also be called "ALPS2B;apoptotic cysteine protease;apoptotic protease Mch-5;CAP4;Casp-8;caspase 8, apoptosis-related cysteine peptidase;caspase 8, apoptosis-related cysteine protease;caspase-8;FADD-homologous ICE/CED-3-like protease;FADD-like ICE;FLICE;ICE-like apoptotic protease 5;MACH;MACH-alpha-1/2/3 protein;MACH-beta-1/2/3/4 protein;MCH5;MORT1-associated ced-3 homolog." in publications.
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What is the shipping cost for "Caspase-8 (P18) Polyclonal Antibody (OABB00267)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Caspase-8 (P18) Polyclonal Antibody (OABB00267)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Caspase-8 (P18) Polyclonal Antibody (OABB00267)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "55391 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Caspase-8 (P18) Polyclonal Antibody (OABB00267)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CASP8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CASP8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CASP8"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CASP8"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CASP8"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CASP8"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.