Caspase-1(p20) Polyclonal Antibody (OABB00394)

Catalog No: OABB00394 (Formerly GWB-BBP317)

Size:100UG

Lane 1: Jurkat Cell Lysate
Lane 2: Raji Cell Lysate
Lane 3: CEM Cell Lysate
Lane 4: PC-12 Cell Lysate

IHC(P): Human Mammary Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Caspase 1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
• Reference: 1. Cerretti, D. P., Hollingsworth, L. T., Kozlosky, C. J., Valentine, M. B., Shapiro, D. N., Morris, S. W.,
  Nelson, N.Molecular characterization of the gene for human interleukin-1-beta converting enzyme
  (IL1BC).Genomics 20: 468-473, 1994.
  2. Thornberry, N. A., Bull, H. G., Calaycay, J. R., Chapman, K. T., Howard, A. D., Kostura, M. J., Miller,
  D. K., Molineaux, S. M., Weidner, J. R., Aunins, J., Elliston, K. O., Ayala, J. M., Casano, F. J., Chin,
  J., Ding, G. J.-F., Egger, L. A., Gaffney, E. P., Limjuco, G., Palyha, O. C., Raju, S. M., Rolando, A. M.,
  Salley, J. P., Yamin, T.-T., Lee, T. D., Shively, J. E., MacCross, M., Mumford, R. A., Schmidt, J. A.,
  Tocci, M. J.A novel heterodimeric cysteine protease is required for interleukin-1-beta processing in
  monocytes.Nature 356: 768-774, 1992
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Caspase-1(CASP1) detection. Tested with WB, IHC-P in Human.

Target Info

• Gene Symbol: CASP1
• Gene Full Name: caspase 1
• Alias Symbols: CASP1 nirs variant 1;caspase 1, apoptosis-related cysteine peptidase;caspase-1;caspase-1 isoform alpha;ICE;IL-1 beta-converting enzyme;IL1BC;IL1B-convertase;interleukin 1, beta, convertase;interleukin 1-B converting enzyme;Interleukin-1 beta convertase;Interleukin-1 beta-converting enzyme;P45.
• NCBI Gene Id: 834
• Protein Name: Caspase-1
• Description of Target: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
• Uniprot ID: P29466
• Molecular Weight: 45159 MW