CASP8AP2 Antibody (OAPB00106)

Catalog No: OAPB00106 (Formerly GWB-DC15D0)

Size:100UG

Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/mL.

Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.

Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. FLASH Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: FLASH antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid. The immunogen is located within the last 50 amino acids of FLASH.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-CASP8AP2 (OAPB00106) antibody is Catalog # OEPB02228
• Application Info: FLASH antibody can be used for detection of FLASH by Western blot at 0.25 to 0.5 ug/ml. An approximate 220 kDa band can be detected. (Optimal dilution should be determined by user)
• Reference: Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85 ;Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3 ;Medema JP. Apoptosis. Life and death in a FLASH. Nature 1999;398:756-7 (WD0800) ;

Target Info

• Gene Symbol: CASP8AP2
• Gene Full Name: caspase 8 associated protein 2
• Alias Symbols: CASP8-associated protein 2;CED-4;FLASH;FLASH homolog RIP25;FLICE associated huge;FLICE-associated huge protein;human FLASH;RIP25.
• NCBI Gene Id: 9994
• Protein Name: CASP8-associated protein 2|Homo sapiens FLASH mRNA, partial cds
• Description of Target: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.
• Uniprot ID: Q9UKL3
• Protein Accession #: NP_001131139.1; NP_001131140.1; NP_036247.1
• Nucleotide Accession #: https://www.ncbi.nlm.nih.gov/nuccore/AF165161
• Molecular Weight: 220 kDa