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CASP8 ELISA Kit (Mouse) (OKEH03437)

Catalog#: OKEH03437
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityMouse
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology CASP8 ELISA Kit (Mouse) (OKEH03437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Casp8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Casp8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Casp8 captured in well.
Kit Range0.156 - 10ng/mL
Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Kit ReproducibilityMean Intra-assay CV%: <= 5.1% (n = 20)
Mean Inter-assay CV%: <= 7.7% (n = 20)
Kit Component
Anti-Casp8 Microplate96 Wells (12 x 8 Well strips)
Casp8 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Casp8 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.093 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Mouse Caspase-8
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCASP8
Official Gene Full Namecaspase 8
Alias SymbolsCASP-8, Caspase-8, FLICE, MACH, Mch5
NCBI Gene Id12370
Protein NameCaspase-8(CASP-8)
Description of TargetMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
Swissprot IdO89110
Protein Accession #NP_001073595.1
Nucleotide Accession #NM_001080126.1
Protein Size (# AA)480
Molecular Weight55 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Casp8.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Casp8.
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