CASP5 Antibody - middle region (OAAB00965)

Catalog No: OAAB00965

Size:400ul

CASP5 Antibody (Center) western blot analysis in MDA-MB231 cell lysates (35ug/lane).This demonstrates the CASP5 antibody detected the CASP5 protein (arrow).

CASP5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC, IHC-P, WB
  Function: Mediator of programmed cell death (apoptosis).
  Tissue Location: Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CASP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the Central region of human CASP5.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50

Target Info

• Gene Symbol: CASP5
• Alias Symbols: ICH-3, ICEREL-III, ICE(rel)III
• NCBI Gene Id: 838
• Protein Name: Caspase-5
• Description of Target: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene.
• Uniprot ID: P51878
• Protein Accession #: NP_001129582.1
• Molecular Weight: 50 kDa