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CASP3 ELISA Kit (Mouse) (OKEH02987)

96 Wells
$469.00
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Gene Symbol:
CASP3
Official Gene Full Name:
caspase 3
NCBI Gene Id:
12367
Alias Symbols:
A830040C14Rik, AC-3, Apopain, CASP-3, Caspase-3, CC3, Cpp32, CPP32, CPP-32, Cysteine protease CPP32, Lice, LICE, mldy, Protein Yama, SCA-1, SREBP cleavage activity 1, Yama
Description of Target:
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Protein Name:
Caspase-3
Swissprot Id:
P70677
Protein Accession #:
NP_001271338.1
Nucleotide Accession #:
NM_001284409.1
Predicted Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.078 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 4.3% (n = 20)
Mean Inter-assay CV%: <= 6.9% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology CASP3 ELISA Kit (Mouse) (OKEH02987) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Casp3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Casp3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Casp3 captured in well.
Kit Component:
ComponentAmount
Anti-Casp3 Microplate96 Wells (12 x 8 Well strips)
Casp3 Lyophilized Standard2
100X Biotinylated Casp3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Casp3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Casp3.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural mouse CASP-3. No significant cross-reactivity or interference was observed.
Publications:

Du, E; McAllister, P; Venna, VR; Xiao, L; Clinically Relevant Concentrations of Ketamine Inhibit Osteoclast Formation In Vitro in Mouse Bone Marrow Cultures. 118, 914-923 (2017). Mouse 27775174

Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
31 kDa
Protein Size (# AA):
277

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