Size:96 Wells
$379.00
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SKU
OKEH02987
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CASP3 ELISA Kit (Mouse) (OKEH02987)

Catalog#: OKEH02987
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityMouse
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology CASP3 ELISA Kit (Mouse) (OKEH02987) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Casp3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Casp3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Casp3 captured in well.
Kit Range0.156 - 10ng/mL
Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Kit ReproducibilityMean Intra-assay CV%: <= 4.3% (n = 20)
Mean Inter-assay CV%: <= 6.9% (n = 20)
Kit Component
ComponentAmount
Anti-Casp3 Microplate96 Wells (12 x 8 Well strips)
Casp3 Lyophilized Standard2
100X Biotinylated Casp3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Mouse Caspase-3
Publications

Du, E; McAllister, P; Venna, VR; Xiao, L; Clinically Relevant Concentrations of Ketamine Inhibit Osteoclast Formation In Vitro in Mouse Bone Marrow Cultures. 118, 914-923 (2017). Mouse 27775174

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCASP3
Official Gene Full Namecaspase 3
Alias SymbolsA830040C14Rik, AC-3, Apopain, CASP-3, Caspase-3, CC3, Cpp32, CPP32, CPP-32, Cysteine protease CPP32, Lice, LICE, mldy, Protein Yama, SCA-1, SREBP cleavage activity 1, Yama
NCBI Gene Id12367
Protein NameCaspase-3
Description of TargetInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Swissprot IdP70677
Protein Accession #NP_001271338.1
Nucleotide Accession #NM_001284409.1
Protein Size (# AA)277
Molecular Weight31 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Casp3.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Casp3.
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