CASP3 Antibody (Phospho-Ser12) (OAAB16261)

Catalog No: OAAB16261

Size:400ul

Dot blot analysis of Phospho-mouse CASP3-S12 Antibody Phospho-specific Pab (Cat. # OAAB16261) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: DB
• Additional Information: Precautions: Phospho-mouse CASP3-S12 is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-l-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity) Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes
  Cellular Location: Cytoplasm.
  Tissue Location: Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This mouse CASP3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of mouse CASP3.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: DB: 1:500

Target Info

• Gene Symbol: Casp3
• Alias Symbols: Ya, AC-, CC3, CPP, mld, AC-3, Casp, Lice, Yama, mldy, CPP32, SCA-1, CASP-3, CPP-32, Caspase-3, A830040C14Rik
• NCBI Gene Id: 12367
• Protein Name: Caspase-3
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P70677
• Protein Accession #: NP_033940.1
• Molecular Weight: 31475 Da