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Catalog No: OASG01029
Size:100 uL
Price: $239.00
SKU
OASG01029
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsPrintable datasheet for anti-CASP3 (OASG01029) antibody
Product Info
Predicted Species ReactivityHuman, Mouse, Rat
Product FormatLiquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationWB, IHC
::Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Sequence & Domain Family: Belongs to the peptidase C14A family.
Cellular Localization: Cytoplasm.
Reconstitution and StorageStore at -20C. Avoid repeated freeze/thaw cycles.
ImmunogenSynthesized peptide derived from the Internal region of human Caspase-3 p17. Location: 100-180aa.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Target Post-Translational ModificationCleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. / S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
SpecificityCleaved-Caspase-3 p17 (D175) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p17 protein resulting from cleavage adjacent to D175.
Intended UseFor research use only.
Application InfoWB: 1:500~2000
IHC: 1:100~300
ELISA: 1:20000
Gene SymbolCASP3
Gene Full Namecaspase 3, apoptosis-related cysteine peptidase
Alias SymbolsCPP32, SCA-1, CPP32B
NCBI Gene Id836; 12367; 25402
Description of TargetInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. / Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. / Inhibited by isatin sulfonamides.
Uniprot IDP42574, P70677, P55213
Molecular Weight35/17 kDa
  1. What is the species homology for "CASP3 Antibody (Cleaved-Asp175) (OASG01029)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".

  2. How long will it take to receive "CASP3 Antibody (Cleaved-Asp175) (OASG01029)"?

    This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".

  3. What buffer format is "CASP3 Antibody (Cleaved-Asp175) (OASG01029)" provided in?

    This item is provided in "Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "CASP3 Antibody (Cleaved-Asp175) (OASG01029)"?

    This target may also be called "CPP32, SCA-1, CPP32B" in publications.

  5. What is the shipping cost for "CASP3 Antibody (Cleaved-Asp175) (OASG01029)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "CASP3 Antibody (Cleaved-Asp175) (OASG01029)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "CASP3 Antibody (Cleaved-Asp175) (OASG01029)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "35/17 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "CASP3 Antibody (Cleaved-Asp175) (OASG01029)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CASP3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CASP3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CASP3"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CASP3"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CASP3"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CASP3"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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