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Catalog No: OKCD03353
Size:96WELLS
Price: $800.00
SKU
OKCD03353
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CASP14 Chemi-Luminescent ELISA Kit (Human) (OKCD03353)

Datasheets/ManualsPrintable datasheet for CASP14 Chemi-Luminescent ELISA Kit (Human) (OKCD03353)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Caspase 14 (CASP14). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Caspase 14 (CASP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Caspase 14 (CASP14) level in the sample or standard.
ELISA Kit Range27.4-20,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 14 (CASP14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 14 (CASP14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CASP14 Microplate96 Wells (12 x 8 Well strips)
CASP14 Lyophilized Standard2
100X CASP14 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.312 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCASP14
Gene Full Namecaspase 14
Alias Symbolsapoptosis-related cysteine protease;ARCI12;CASP-14;caspase 14, apoptosis-related cysteine peptidase;caspase 14, apoptosis-related cysteine protease;caspase-14.
NCBI Gene Id23581
Protein NameCaspase-14
Description of TargetNon-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).
Uniprot IDP31944
Protein Accession #NP_036246
Nucleotide Accession #NM_012114
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