CASP12 Antibody - middle region (OAAB08271)

Catalog No: OAAB08271

Size:400ul

CASP12 Antibody (Center) (Cat. #OAAB08271) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CASP12 antibody detected the CASP12 protein (arrow).

CASP12 Antibody (Center) (Cat. #OAAB08271)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CASP12 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

CASP12 Antibody (Center) (Cat. #OAAB08271) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CASP12 Antibody (Center)(Cat#OAAB08271) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC, IF
  Function: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections Reduces activation of NF-kappa-B in response to TNF.
  Tissue Location: Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CASP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the Central region of human CASP12.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: CASP12
• Alias Symbols: CASP-12, CASP12P1
• NCBI Gene Id: 100506742
• Protein Name: Inactive caspase-12
• Description of Target: Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene.
• Uniprot ID: Q6UXS9
• Protein Accession #: NP_001177945.1
• Molecular Weight: 39 kDa